Bzr1 chip-seq
WebNov 16, 2010 · Genome-Wide Analysis of In Vivo BZR1-Binding Sites. To identify genes directly regulated by BR signaling, we performed a ChIP-chip analysis using a polyclonal anti-YFP antibody and transgenic Arabidopsis plants expressing the BZR1-CFP fusion protein from the BZR1 promoter (He et al., 2005, Wang et al., 2002), with wild-type … WebNov 27, 2024 · Yeast cells with positive DNA-protein interactions were grown on Leu − plates with 100 ng mL −1 aureobasidin A. C, Direct binding of BZR1 to the promoters of ATG2 and ATG6 was investigated using ChIP-qPCR in BZR1OE plants. Six-week-old BZR1OE plants were treated with water or 500 n m BL, and input chromatin was isolated …
Bzr1 chip-seq
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WebCRY1a-OE, HY5-OE, and BZR1-OE transgenic plants; HY5-RNAi plants; and bzr1 mutant were generated as described previously (49–55). The CRISPR/Cas9 mutants of hy5 and brc1 (brc1 #5, brc1 #9 lines) in the cv. Condine Red background were generated similar to previous studies (20, 36). The mutation sites are shown in SI Appendix, Figs. S4A and S7A. WebMay 27, 2014 · ChIP-Seq analysis R (CSAR) software was used to identify binding peaks, with parameters (backg = 10, norm = −1, test = 'Ratio', …
WebJul 22, 2012 · Among the six PRE members, PRE1, PRE5 and PRE6 were identified as PIF4 targets in the ChIP-seq analysis (Supplementary Fig. S6a), and confirmed by ChIP–qPCR to be direct targets of both BZR1 and ... WebSep 13, 2024 · To test whether BES1 and BZR1 can directly regulate the expression of DYT1, we carried out a chromatin immunoprecipitation (ChIP) analysis to investigate whether BES1 and BZR1 can be enriched in ...
WebMar 11, 2005 · Lane S is the G + A chemical sequencing reaction. Footprint regions are marked by vertical lines and corresponding sequence. (D) RBSS for optimal BZR1 binding site. Shown on the top is the alignment of all identified sequences, with the matching sequences marked in red. ... we performed chromatin immunoprecipitation (ChIP) … Web总 RNA 提取和 qRT-PCR 分析\RNA-Seq. Western Blot\重组蛋白纯化和 EMSA\染色质免疫沉淀 (ChIP)-qPCR. 双荧光素酶测定\酵母单杂交试验 ... ( C ) WT 植物、 bzr1和hy5突变体侧芽中BRC1的转录水平。 ...
WebApr 14, 2024 · The PIF4–BZR1 complex tends to directly activate genes involved in cell elongation and responses to auxin and GA but indirectly represses genes encoding photosynthetic proteins. ... The integration of …
WebLists of BR- and auxin-responsive genes and BZR1 target genes. Comparison of genes up- or down-regulated by BR or auxin, identified by RNA-Seq in roots (Table 2-S1), to the BZR1 ChIP-chip and ChIP-Seq data [S1, S2], and the RootMap spatial gene expression data [S3]. QC, quiescent center; MZ, meristem zone; TEZ, transition-elongation zone; LEZ ... riding lawn mower craigslistWebJul 22, 2012 · The DNA-bound proteins were immunoblotted using anti-MBP antibody. (i) GA increases the level of BZR1–DNA binding in vivo. ChIP was performed using anti-YFP antibodies followed by qPCR analysis. riding lawn mower craigslist gilroy caWebApr 20, 2015 · Comparison of genes upregulated or downregulated by BR or auxin, identified by RNA-seq in roots (Table S1), to the BZR1 ChIP-chip and ChIP-seq data, and the RootMap spatial gene expression data. QC, quiescent center; MZ, meristem zone; TEZ, transition-elongation zone; LEZ, late elongation zone; DZ, differentiation zone. riding lawn mower craftsman lt1000riding lawn mower cup holder craftsmanWeb微信公众号植物生物技术Pbj介绍：本公众号致力于植物生物技术领域重要进展的推介，由 Plant Biotechnology Journal杂志（2024年影响因子13.3，植物学研究型Top3 期刊）官方授权，华中农业大学为运营主体，PBJ杂志执行主编金双侠教授担任责任编辑；JIPB 浙江大学汪俏梅课题组揭示油菜素甾醇促进番茄果实 ... riding lawn mower cyber mondayWebChIP-Seq and RNA-Seq experiments show that ARF6, PIF4, and BZR1 bind to largely overlapping targets in the genome and synergistically activate gene expression. In vitro … riding lawn mower custom exhaustWebTo generate comparable data sets, we performed BZR1 ChIP-Seq analysis using dark-grown seedlings (Figure 1—source data 1B), as used in the ARF6 and PIF4 ChIP-Seq (Oh et al., 2012). Interestingly, large portions of ARF6 binding targets overlapped with either tar-gets of BZR1 (51%) or PIF4 (71%) or both (42%) (Figure 1C). The common targets ... riding lawn mower cutting grass videos