Glycolytic proton efflux rate
WebThe Glycolytic Proton Efflux Rate (glycoPER) was converted from OCR and ECAR data through Seahorse XF e 24 Glycolytic Rate Assay Report. Data from three separate … WebApr 13, 2024 · Glycolytic proton efflux rate (glycoPER) is the rate of proton efflux into the extracellular solution during glycolysis. Its value is equal to the value of PER multiplied by the percentage of glycolytic PER (basal) measured by seahorse XF24 analyzer. HUVEC and HepG 2 cell viability assay.
Glycolytic proton efflux rate
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WebMay 6, 2024 · An automated Glycolytic Rate Assay utilized both the OCR and the extracellular acidification rate (ECAR) to calculate the glycolytic proton efflux rate (PER). After the basal glycolytic PER was measured, the compensatory glycolysis was measured by inhibiting oxidative phosphorylation by injecting the complex I and II inhibitors … WebMar 31, 2016 · This ambiguity leads to a rate of total extracellular acidification that can be greater than glycolytic rate to lactate, because it is contaminated to varying degrees (ranging from 0 to 100%) by protons derived from respiratory CO 2 production.
WebFeb 15, 2024 · b, Glycolytic proton efflux rate (glycoPER) in 4T1-KD and 4T1-Sc cultures (n = 20). 2-DG, 2-deoxy-d-glucose; Rot/AA, rotenone plus antimycin A. c, In vivo growth of 4T1-KD and 4T1-Sc tumours ... WebApr 1, 2024 · Bic suppressed the oxygen consumption rate, extracellular acidification rate, and mitochondrial proton efflux rate, downregulated in vivo but upregulated in vitro glucose transporter (GLUT)-1, reduced ... Seahorse XF glycolytic rate assay of the respiratory and metabolic characteristics in rat mesangial cells (RMCs) affected by 0 (Control), 30 ...
WebDec 22, 2024 · The total Proton Efflux Rate (PER) and the Glycolytic Proton Efflux Rate (GlycoPER) were measured using both OCR and ECAR values to account for mitochondrial (CO 2) acidification from the mitochondrial TCA cycle ( … WebThe Glycolysis Rate Assay measured glycolytic rates for basal conditions and compensatory glycolysis following mitochondrial inhibition after the injection of 0.5 mM R/A. The final injection is 2-deoxy-glucose (2-DG), a glucose analogue, which inhibits glycolysis through competitive binding with glucose hexokinase. ... PER (proton efflux rate ...
WebJun 17, 2024 · The y-axis represents the glycolytic proton efflux rate (PER) at the basal and compensatory level. Statistical significance among groups (* P < 0.05, ** P < 0.01) was determined by a non ...
Web(D) The Glycolytic Rate Assay was used to measure glycolysis as well as compensatory glycolysis of HEK293 MUL1 (+/+) and HEK293 MUL1 (−/−) cells. The Glycolytic Proton Efflux Rate (glycoPER)... scannerizzare foto windows 10WebMar 21, 2024 · (C and D) Glycolytic proton efflux rate (glycoPER) analyses of WT and GLUT3-deficient Th1 (C) and Th17 (D) cells using a Seahorse extracellular flux analyzer; mean ± SEM of 5 mice. ... To further test whether GLUT3-deficient T cells also have an impaired glycolytic flux, we analyzed the proton efflux rate (PER) as a measure for … ruby red leather blazerWebGlycemic load. The glycemic load (GL) of food is a number that estimates how much the food will raise a person's blood glucose level after eating it. One unit of glycemic load … ruby red linzi glassruby red kolsch beerWebNov 24, 2024 · Glycolytic proton efflux rate (glycoPER) and oxygen consumption rate (OCR) were determined by Seahorse technology (Agilent Technologies, Santa Clara, … ruby red ladies topsWebAug 8, 2024 · Total proton efflux rate (PER) and glycolytic proton efflux rate (glycoPER) were determined by measuring the OCR and ECAR under basal conditions and after sequential injections of rotenone/antimycin A (R/A, 1 μM each) and 2-DG (50 mM). The data were normalized to cell numbers and the proton efflux rate were calculated using the … ruby red kolsch nutrition factsWebSimilarly, basal proton efflux rate (PER, the measure of extracellular acidification, Fig. 5d), but not the PER derived from glycolysis ( Fig. 5e), was increased in PD astrocytes. scanner jam brother