Human thp-1 monocytes
Web18 okt. 2024 · THP-1 is a human monocytic cell line isolated from a patient with acute monocytic leukemia. This cell line can be differentiated into macrophages and are widely used to study monocyte and macrophage functions and in immune modulation studies ( 19 ). Web5 nov. 2024 · Recently, the widely used monocytic leukemia cell line, THP-1, was comprehensively examined to understand mechanistic relationships between the 3D …
Human thp-1 monocytes
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Web29 nov. 2024 · Our experiments were carried out on murine macrophage cell line (BMDM) and human monocytes cell line (THP-1) using IFNβ as a IFNAR ligand. We demonstrate that Pellino3 is important for IFNβ-induced phosphorylation and nuclear translocation of STAT1/STAT2/IRF9 complex which interacts with CXCL10 promoter and enhances its … Web5 jun. 2015 · The monocytic cell line THP-1 was maintained in Roswell Park Memorial Institute medium (RPMI 1640) supplemented with 10% fetal calf serum at 37 °C and 5% CO 2. Differentiation of THP-1 cells was induced by treatment with 50 nM phorbol 12-myristate 13-acetate (PMA) for 24 to 72 h.
Web7 aug. 2008 · Auwerx, J. The Human Leukemia Cell Line, THP-1: A Multifacetted Model for the Study of Monocyte-Macrophage Differentiation. Experientia 1991, 47, 22–31. [Google Scholar] Auwerx, J; Staels, B; Van Vaeck, F; Ceuppens, JL. Changes in IgG Fc Receptor Expression Induced by Phorbol 12-Myristate 13-Acetate Treatment of THP-1 Monocytic … WebTHP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder research, immunology …
WebTHP1-Blue™ were derived from the human THP-1 monocyte cell line by stable integration of an interferon regulatory factor (IRF)-inducible SEAP reporter construct. As a result, … WebTHP-1 is a human leukaemia monocytic cell line from the peripheral blood of a 1 year old human male. After exposure to phorbol-12-myristate-13-acetate (PMA), THP-1 cells in …
Web18 nov. 2015 · Pession A, Martino V, Tonelli R, Beltramini C, Locatelli F, Biserni G, Franzoni M, Freccero F, Montemurro L, Pattacini L, Paolucci G. MLL-AF9 oncogene expression …
Webacute monocytic leukaemia. THP-1 cells resemble primary monocytes and macro-phages in morphology and differentiation properties. THP-1 cells show a large, round single-cell … dillon sheridanWeb27 sep. 2024 · Ushashi Banerjee. 1) Thawing the vial in 37C water bath, then spinning down at ~800rpm for 3 minutes. Then decant the FBS, resuspend in pre-warmed media and … dillon sheriff department scWebTHP1 is a monocytic cell line and monocytes usually have low to minimal phagocytic capacity. You may differentiate monocytes to macrophages using 100-150 nM PMA and … dillons harry and edgemoor wichita ksWebArticle Snippet: Human immortalized monocyte-like leukemia THP-1 cells (ATCC ® TIB-202™; American Type Culture Collection, Manassas, VA, USA) were placed in a 6-well … dillons harley davidson omaha neWeb14 mrt. 2008 · THP-1 monocytic cells have been extensively characterized, and they display phenotypic and functional features of mature human monocytes, including enhanced … for the movies buckcherryWebAbstract. Monocytes play a central role in the response of tissues to biomaterials. Monocytic cell lines such as the THP-1 cell line have been used extensively as models … dillons harry and webb wichitaWebBackground: Phorbol 12-myristate 13-acetate (PMA)-induced differentiation of human monocytic THP-1 cells is an experimental model for preparing resting macrophages (M0) for cell polarization... dillon shields