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Nphs2-cre

Web19 aug. 2024 · 中华儿科杂志2024年第9期遗传性肾病综合征小鼠模型的研究现状. 本论文发表于中华儿科杂志，属于医学相关论文范文材料。. 仅供大家论文写作参考。. ‑00432原发性肾病综合征（nephroticsyndrome，NS）是儿童常见的肾小球疾病，发病率因人种和地区而异，每年（1.15 ... Web29 apr. 2024 · To further probe these NPHS2-Cre-dependent changes in podocytes and GBM thickness, RNA sequencing was performed on isolated glomeruli of NPHS2-Cre …

Podocyte-Specific Expression of Cre Recombinase in Transgenic …

Web7 sep. 2024 · The CD16/7-nephrin activation model allows for precisely-timed induction of nephrin tyrosine phosphorylation in live cells by addition of specific antibodies that cluster the extracellular CD16 domain and results in intracellular tyrosine phosphorylation of nephrin’s cytoplasmic tail. Web9 sep. 2024 · The NPHS2-Cre transgene comprises the Cre coding sequence under the control of 2.5 kb of the human NPHS2 promoter. The NPHS2 gene encodes Podocin, which is specifically expressed in podocytes. In their paper, Balkawade et al. observed a thickening of the glomerular basement membrane (GBM) in 24-week-old NPHS2-Cre mice, which … davey and goliath characters https://maamoskitchen.com

Single-cell transcriptomics reveals a mechanosensitive injury …

Web13 okt. 2024 · The Cre-Lox recombination system enables the generation of tissue-specific or inducible knockouts with a high level of control over the spatial and temporal expression of genes. Figure 1. Mechanism of Cre-LoxP System. (A) An overview of Cre-LoxP system. 38 kDa Cre recombinase recognizes the loxP sites of specific 34 bp DNA sequences, … WebThe Jackson Laboratory nphs2 also known as podocin cre mice. Nphs2 Also Known As Podocin Cre Mice, supplied by The Jackson Laboratory, used in various techniques. … Web(NPHS2-Cre) to generate a conditional knockout. By conventional structural and functional measures (histology by periodic acid-Schiff staining, albuminuria, and plasma creatinine), we did not detect gasbuddy great falls montana

Podocyte-specific Crb2 knockout mice develop focal segmental

Category:乘风破浪的大鼠——如何突破技术限制再次发挥在特定领域的研究 …

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Nphs2-cre

cre小鼠是什么？cre工具鼠详细介绍 / 开普饭

Web19 mrt. 2024 · For rapamycin treatment, Nphs2-Cre ( n = 17), Tsc2flox/flox ( n = 16), and Tsc2Δpodocyte ( n = 8) mice were intraperitoneally injected with rapamycin (LC Laboratories, Woburn, MA) at 2 mg kg -1 of body weight every other day … WebOf note, 2 out of 17 analyzed 2.5P-Cre/ROSA26 mice of founder line #295 did not express significant amounts of -gal in the kidneys. Thus, penetrance of Cre expres-FIG. 1. Transgene construct. A 2.5-kb fragment of the human NPHS2 5 flanking region containing the entire 5 untranslated re-gion was placed in front of a Cre recombinase cassette ...

Nphs2-cre

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Web10 jan. 2024 · scRNA-seq of BTBR ob/ob mouse kidneys with early DN. We generated BTBR ob/ob podocyte-reporter mice (BTBR.Cg-Lep ob/ob WiscJ;Gt (ROSA)26Sor tm4(ACTB−tdTomato,−EGFP)Luo;Tg(NPHS2-cre) 295Lbh; hereafter referred to as BTBR ob/ob or DN mice) to investigate the cellular and molecular changes that occur in DN. By … Web实验小鼠是目前应用最为广泛的一种实验动物，在探寻基因基础功能，疾病发病机制以及药物临床前筛选等方面有着十分重要的作用。 其原因在于小鼠和人的基因具有极高的相似度（小鼠99%的基因能在人类基因组中找到同源…

WebTwenty- four transgenic founders were generated in which Cre recombinase was placed under the regulation of a 2.5-kb fragment of the human NPHS2 promoter. Previously, this … Web28 okt. 2024 · The epidermal growth factor receptor (EGFR) is expressed in numerous cell types in the adult mammalian kidney and is activated by a family of EGF-like ligands. EGFR activation has been implicated in a variety of physiologic and pathophysiologic functions. There is increasing evidence that aberrant EGFR activation is a mediator of progressive …

Web本模型可利用Cre/loxP位点特异性重组系统对细胞命运进行追踪展示，揭示特定类型细胞在发育、疾病中的再生、自我更新、分化过程。 双荧光报告基因系统提供zsGreene及tdTomato两种荧光标记，实现了重组/非重组细胞的实时检测，因此，本模型也是一种示踪特异性Cre表达位置的理想工具。 图二 B6-G/R与Cre小鼠交配后部分组织荧光表达检测 好了~以上只是 … WebA recent article described a thickening of the glomerular basement membrane (GBM) along with changes in the expression of key components of the extracellular matrix in 6-month …

Web赛业生物可为您提供基因敲除、点突变、基因敲入等多种基因编辑大鼠服务，还可为您提供SD、Wistar、Long Evans、F344、Brown Norway大鼠等多种品系选择，致力于为您构建更好的大鼠模型。. 欢迎在线咨询或拨打400-680-8038联系我们。. 使用Cre/loxp系统建立条件 …

WebFirst, Nphs2 encodes podocin, an essential component of the slit diaphragm, a cell-cell junctional structure between adjacent podocytes, which is one of the most important components of the barrier that prevents proteins from leaving the circulation during filtration. gas buddy greenville scWebNPHS2-cre. 2024-11-19. B6.Cg-Tg(NPHS2-cre)295Lbh. 品系编号：GAP1021. 品系简称：NPHS2-cre. 品系特点：. NPHS2-Cre 小鼠可存活且可生育，Cre 重组酶的表达通过人 … davey and goliath dvd box setWeb008205 B6.Cg-Tg(NPHS2-cre)295Lbh/J These transgenic mice (commonly referred to as podocin-Cre mice) have expression of Cre recombinase directed to podocytes within kidney glomeruli by the human podocin ( NPHS2 ) promoter/enhancer region and may be useful in generating Cre-lox conditional knockouts for studying the role of podocyte nephrobiology … davey and goliath cousin barneyWebindividual cell types. To further validate the clustering analysis results, we used Nphs2. Cre. mTmG, Scl. Cre. mTmG and Cdh16. Cre. mTmG mice as reporter lines to mark podocytes, endothelium and tubule cells. The GFP expression in these models confirmed the proposed cell identity of our cell clusters (fig. S8 gasbuddy greenville wiWeb008523 129S6.Cg-Tg(NPHS2-cre)295Lbh/BroJ These 129S6 Podocin-Cre mice (harboring the p2.5P-Cre transgene) have expression of Cre recombinase directed to podocytes … gas buddy griffin gaWeb21 mrt. 2024 · NPHS2 (NPHS2 Stomatin Family Member, Podocin) is a Protein Coding gene. Diseases associated with NPHS2 include Nephrotic Syndrome, Type 2 and … davey and goliath down on the farmWebNphs2.Cre + ;Coq6 flox/flox mutant mice show (A) reduced podocin staining (arrows) appearing only on a few capillary loops (arrow head), whereas (B) nephrin expression is … gas buddy grimes iowa