WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ... Webb酵母是单细胞低等的真核生物，具有生长快、成本低、易于遗传操作，能对外源蛋白进行翻译后加工和修饰、不产生有毒产物等特点，认为是外源蛋白的合适宿主 [1] 。 酵母分为三类：第一是酿酒酵母；第二是栗酒裂殖酵母；第三是非常规酵母，主要有巴氏毕赤酵母、乳酸克鲁维亚酵母等 [2] 。

Addgene: Vector Database - pHIS-2

Webb1 dec. 2024 · AD/pATM1-pHIS2 and AD-ATM1/pATM1-pHIS2 were cotransformed into the Y187 strain and cultured on SD/-Trp/-Leu for 2–3 days at 30 °C. Then, healthy and consistent clones were diluted, ... Webb1、pHis2-Bait或pAbAi-Bait和AD-prey质粒 2、或者Bait和Prey序列，质粒由我们构建 1、提供详细的、完整的实验报告，清晰的实验图片 2、在受体菌的阳性菌 自激活和毒性验证: … marie bui thi thuan antibes

酵母单杂交.ppt-原创力文档

WebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. WebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community … Webb载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit. It contains a HIS3 nutritional reporter gene, located downstream of a multiple cloning ... naturalisation certificate meaning